Development of technology for production of virus free plantlets of sweet potato using the shoot tip culture

The present study has been carried out to develop virus free plantlets of sweet potato using the shoot tip culture.The sweet potato variety Hongguniang 2 was used as experimental materials to study effects of various concentrations and combinations of 6-BA,NAA and GA3,size of the shoot tip and soaking at different temperature for different times on virus free shoot tip culture.The virus in regenerating plantlet clones was detected using indicator plant method.The results showed that different combinations with different concentrations of 6-BA,NAA,GA3 in medium had significant effect on the induction of adventitious buds and roots in variety Hongguniang 2.The best medium for induction of callus and cluster buds was found to be MS + 1.0 mg / L 6-BA+ 0.01 mg / L NAA + 0.1 mg / L GA3.The optimum medium for the rooting was 1 / 2MS + 0.2 mg / L NAA.Further,the average virus-free plantlet production rate was found to be 96.7% for the tested 90 plantlet clones.