Effect of ziprasidone on the proliferation and IL-1β expression of N9 cells

Objective To investigate the effect of ziprasidone on the proliferation and IL-1β expression of N9 cells.Methods There were two kinds of treatments:for derect effect,N9 cells were treated with different concentrations of ziprasidone(5μm、10μm、20μm、50 μm)and control group(DMEM culture medium containing 4 % fetal calf serum) for 24h and 48h;for protect effect,N9 cells were cultured in DMEM medium without fetal calf serum for 12h after derect treated with different concentrations of ziprasidone and control.The cells viability,the BrdU incorporate and the expression of IL-1β were measured by the cell counting Kit-8(CCK-8) and immunocytochemical method,respectively.Results The cell viability,the BrdU positive cells and the expression of IL-1β in both 20 μm and 10 μm ziprasidone treated groups had significant differences compared to ziprasidone 50 μm,5μm treated group and the control(P 0.01,P 0.05),and there were no significant differences among ziprasidone 50 μm,5μm treated group and the control after treatment for 24h.Similarly,after treatment for 48h,the cell viability,the BrdU positive cells and the expression of IL-1β in both 20 μm and 10 μm ziprasidone treated groups had significant differences compared to ziprasidone 50 μm,5μm treated group and the control(P 0.01),20 μm and 10 μm ziprasidone had protect effect for N9 cells.But the proliferation was inhibited by 50 μm ziprasidone.Conclusion The viability of N9 cells can be improved and protected by ziprasidone without change of the cell shape,and the increase of proliferation and the expression of IL-1β also observed.This may be one of the possible neuro-protective mechanism of ziprasidone.