Effect of olanzapine on PC12 cells from apoptosis by serum-free induced

Objective:To investigate the possible protective effects and related mechanisms of olanzapine on PC12 cells,apoptosis induced by serum-free culture was observed. Method:NGF-derivation PC12 cells were used as the model of neurons for observation,and apoptosis was undertaken by serum-free culture.After PC12 cells were treated with olanzapine,MTT assay was performed to determine cell viability,apoptotic cell ratio and cell cycle were detected with flow cytometer,the morphological changes of PC12 cells were examined with Hoechst33342 staining as well. Results:Cell viability of olanzapine treatment group was significantly higher than control group after 72 h in vitro at the concentration of 100 μM(P0.05),whereas at the concentration of 12.5、 25、 50、 200 μM.Cell viability of haloperidol treatment group,however,was lower than that in control group at the all concentrations.Apoptosis ratios,of serum,control,haloperidol or olanzapine treatment groups were 17.9%、 51.9%、 59.8% or 36.6% respectively,Most cells in control group and haloperidol-treatment group were detained at G_1 stage.Apoptotic cells were fewer in olanzapine-treated group and the majority of cells are characterized by nuclear condensation in Hoechst33342 staining.More apoptotic cells were observed in control group and most of them were characterized by nuclear fragmentation. Conclusion:It was one of the possible neuroprotective mechanisms that olanzapine protected PC12 cells from apoptosis induced by serum-free culture.