ScholarLens Back to search
2014•Zhonghua xingwei yixue yu naokexue zazhiRequires access

Protective effects of ziprasidone on the damage of hippocampal-derived neural stem cell induced by lipopolysaccharide

Bin Xu

Open publisher page 0 citations

Abstract

Objective To investigate the effects of ziprasidone on the hippocampal-derived neural stem cells (NSCs) viability and apoptosis injured.by LPS. Methods The NSCs were derived from the hippocampus of fetal rats, after the primary neurospheres passaged, the cells were treated with LPS (200 μg/L) and different concentrations of ziprasidone for 48 h or 72 h. The cell viability and the level of LDH were measured by the kit of WST-8 and LDH detected kit, respectively. Furthermore, the apoptosis rate of each dosage group was measured by using Annexin-V-FLUOS Staining Kit. Results (1) Cell viability test showed that the cell viability of LPS group (OD value, 0.265±0.047) was significantly reduced than that of sham (OD value, 0.380±0.029), LPS+ 5 μM (OD value, 0.316±0.008) and LPS+ 10 μM (OD value, 0.321±0.006) group after ziprasidone treatment for 48 h. There were also significant difference of cell viability between sham, LPS+ 5 μM, LPS+ 10 μM and LPS group after ziprasidone treatment for 72 h. (2) LDH test showed that the LDH level of LPS group was significantly decreased than that of sham, LPS+ 5 μM and LPS+ 10 μM after 48 hour's ziprasidone treatment (P<0.01). The LDH level of LPS group was also significantly reduced than that of sham, LPS+ 5 μM and LPS+ 10 μM after 72 hour's ziprasidone treatment (P<0.01). (3) The apoptosis of LPS+ 10 μM((17.87±2.29)%), LPS+ 5 μM ((18.45±3.84)%) treated group or sham group((9.15±1.54)%) was significantly lower than LPS group((22.67±2.15)%) by using flow cytometry after 48 h treatment.Furthermore, the apoptosis of LPS+ 10 μM ((10.52±1.42)%) treated group or sham group((8.45±1.04)%) was significantly lower than LPS group((15.70±2.97)%) after 72 h treatment. Conclusion The cellular damage of NSCs injured by LPS can be restrained by ziprasidone, and this effect might be one of the cellular mechanism of neural protective effect of ziprasidone. Key words: Ziprasidone; Neural stem cells; Protective effect; Lipopolysaccharide

About this research paper

What this paper is about

Objective To investigate the effects of ziprasidone on the hippocampal-derived neural stem cells (NSCs) viability and apoptosis injured.by LPS. Methods The NSCs were derived from the hippocampus of fetal rats, after the primary neurospheres passaged, the cells were treated with LPS (200 μg/L) and different concentrations of ziprasidone for 48 h or 72 h. The cell viability and the level of LDH were measured by the kit of WST-8 and LDH detected kit, respectively. Furthermore, the apoptosis rate of each dosage group was measured by using Annexin-V-FLUOS Staining Kit. Results (1) Cell viability test showed that the cell viability of LPS group (OD value, 0.265±0.047) was significantly reduced than that of sham (OD value, 0.380±0.029), LPS+ 5 μM (OD value, 0.316±0.008) and LPS+ 10 μM (OD value, 0.321±0.006) group after ziprasidone treatment for 48 h. There were also significant difference of cell viability between sham, LPS+ 5 μM, LPS+ 10 μM and LPS group after ziprasidone treatment for 72 h. (2) LDH test showed that the LDH level of LPS group was significantly decreased than that of sham, LPS+ 5 μM and LPS+ 10 μM after 48 hour's ziprasidone treatment (P<0.01). The LDH level of LPS group was also significantly reduced than that of sham, LPS+ 5 μM and LPS+ 10 μM after 72 hour's ziprasidone treatment (P<0.01). (3) The apoptosis of LPS+ 10 μM((17.87±2.29)%), LPS+ 5 μM ((18.45±3.84)%) treated group or sham group((9.15±1.54)%) was significantly lower than LPS group((22.67±2.15)%) by using flow cytometry after 48 h treatment.Furthermore, the apoptosis of LPS+ 10 μM ((10.52±1.42)%) treated group or sham group((8.45±1.04)%) was significantly lower than LPS group((15.70±2.97)%) after 72 h treatment. Conclusion The cellular damage of NSCs injured by LPS can be restrained by ziprasidone, and this effect might be one of the cellular mechanism of neural protective effect of ziprasidone. Key words: Ziprasidone; Neural stem cells; Protective effect; Lipopolysaccharide

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To investigate the effects of ziprasidone on the hippocampal-derived neural stem cells (NSCs) viability and apoptosis injured.by LPS. Methods The NSCs were derived from the hippocampus of fetal rats, after the primary neurospheres passaged, the cells were treated with LPS (200 μg/L) and different concentrations of ziprasidone for 48 h or 72 h. The cell viability and the level of LDH were measured by the kit of WST-8 and LDH detected kit, respectively. Furthermore, the apoptosis rate of each dosage group was measured by using Annexin-V-FLUOS Staining Kit. Results (1) Cell viability test showed that the cell viability of LPS group (OD value, 0.265±0.047) was significantly reduced than that of sham (OD value, 0.380±0.029), LPS+ 5 μM (OD value, 0.316±0.008) and LPS+ 10 μM (OD value, 0.321±0.006) group after ziprasidone treatment for 48 h. There were also significant difference of cell viability between sham, LPS+ 5 μM, LPS+ 10 μM and LPS group after ziprasidone treatment for 72 h. (2) LDH test showed that the LDH level of LPS group was significantly decreased than that of sham, LPS+ 5 μM and LPS+ 10 μM after 48 hour's ziprasidone treatment (P<0.01). The LDH level of LPS group was also significantly reduced than that of sham, LPS+ 5 μM and LPS+ 10 μM after 72 hour's ziprasidone treatment (P<0.01). (3) The apoptosis of LPS+ 10 μM((17.87±2.29)%), LPS+ 5 μM ((18.45±3.84)%) treated group or sham group((9.15±1.54)%) was significantly lower than LPS group((22.67±2.15)%) by using flow cytometry after 48 h treatment.Furthermore, the apoptosis of LPS+ 10 μM ((10.52±1.42)%) treated group or sham group((8.45±1.04)%) was significantly lower than LPS group((15.70±2.97)%) after 72 h treatment. Conclusion The cellular damage of NSCs injured by LPS can be restrained by ziprasidone, and this effect might be one of the cellular mechanism of neural protective effect of ziprasidone. Key words: Ziprasidone; Neural stem cells; Protective effect; Lipopolysaccharide

Key concepts: Ziprasidone, Viability assay, Lipopolysaccharide, Chemistry, Apoptosis, Neural stem cell, Annexin, Hippocampal formation

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Protective effects of ziprasidone on the damage of hippocampal-derived neural stem cell induced by lipopolysaccharide — Research Paper | ScholarLens