ScholarLens Back to search
2003•Journal of Nanhua UniversityRequires access

Construction and Identification of Recombinant Plasmid of NKG2D Gene

Liu Min

Open publisher page 0 citations

Abstract

Objective To construct recombinant vector of NKG2D gene. Methods NKG2D cDNA was obtained from peripheral blood mononuclear cells of human by using RT-PCR and was cloned into pMD18-T. The recombinant vector was identified with double restriction enzymes digestion and DNA sequence analysis. Results The sequence of NKG2D cDNA obtained from the recombinant vector was identical with that published on GeneBank. Conclusion The recombinant vector pMD18-NKG2D was constructed successfully,which lay the foundation for further studying biological activity of NKG2D.

About this research paper

What this paper is about

Objective To construct recombinant vector of NKG2D gene. Methods NKG2D cDNA was obtained from peripheral blood mononuclear cells of human by using RT-PCR and was cloned into pMD18-T. The recombinant vector was identified with double restriction enzymes digestion and DNA sequence analysis. Results The sequence of NKG2D cDNA obtained from the recombinant vector was identical with that published on GeneBank. Conclusion The recombinant vector pMD18-NKG2D was constructed successfully,which lay the foundation for further studying biological activity of NKG2D.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To construct recombinant vector of NKG2D gene. Methods NKG2D cDNA was obtained from peripheral blood mononuclear cells of human by using RT-PCR and was cloned into pMD18-T. The recombinant vector was identified with double restriction enzymes digestion and DNA sequence analysis. Results The sequence of NKG2D cDNA obtained from the recombinant vector was identical with that published on GeneBank. Conclusion The recombinant vector pMD18-NKG2D was constructed successfully,which lay the foundation for further studying biological activity of NKG2D.

Key concepts: Recombinant DNA, Complementary DNA, Molecular biology, Plasmid, Restriction enzyme, Biology, Vector (molecular biology), Peripheral blood mononuclear cell

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Construction and Identification of Recombinant Plasmid of NKG2D Gene — Research Paper | ScholarLens