HP450 Gene Clone and Construction and Identification of the Recombinant Vector pMD18-HP450

Objective To construct cloning vector of HP450 gene.Methods The HP450 gene was obtained by reverse transcriptase-polymerase chain reaction(RT-PCR).It was cloned into pMD18-T vector using T-A clone method.We identified the recombinant plasmidvector pMD18-HP450 by restriction digestion,sequencing and PCR using bacteria as the templet.Results The aim strip appeared after the PCR and the restriction digestion production were logical.The sequencing showed that 100% nucleotides were homologous between aim gene and H1gene in gene bank.Conclusion The HP450 gene had been cloned.The recombinant vector pMD18-HP450 is constructed correctly.