Purification of Total Flavonoids from Leaves of Ficus carica by Macroporous Resin

Objective:To study on purification technology conditions of total flavonoids from leaves of Ficus carica by macroporous resin.Method:Five models of macroporous resins were selected by static adsorption,purification technology of total flavonoids from leaves of F.carica with macroporous resin was optimized by dynamic adsorption.Result:Optimum purification technology was as follows:concentration of sample 2 g·L-1,velocity of sample 2 BV·h-1,volume of sample 3 BV,eluted with 2 BV deionized water and abandoned eluate,then eluted with 4BV 80% ethanol at speed of 2 BV·h-1,eluate was collected;Yield of total flavonoids was 51.3% at this technology conditions,and recovery rate of total flavonoids was 77.6%.Conclusion:D140 macroporous resin could purify total flavonoids from leaves of F.carica effectively.