Study on the resistance of pvl gene-negative MRSA to antimicrobial drugs

Objective To investigate the resistance of panton-valentine leukocidin(PVL) pvl gene-negative methicillin-resistant Staphylococcus aureus(MRSA) to antimicrobial drugs.Methods Cefoxitin disk diffusion test was used to detect MRSA.Polymerase chain reaction(PCR) was used to the detection of mecA gene and pvl gene.The minimum inhibitory concentration(MIC) of MRSA was detected by agar dilution,and the resistance of pvl gene-negative MRSA to 12 antimicrobial drugs was detected.Results 33 pvl gene-negative MRSA were detected from 71 strains of Staphylococcus aureus.The detection rate was 46.4%(33/71).The resistance rate of penicillin was 100%,and other antimicrobial drugs showed multiple drug resistance.The main resistance patterns accounting for 66.7%(22/33) was showed the resistance of ECLOTGMP.The resistant strains to daptomycin,teicoplanin and vancomycin were not found.Conclusions The pvl gene-negative MRSA with resistance to erythromycin,ciprofloxacin,clindamycin,tetracycline,gentamicin,sulphamethoxazole,oxacillin and penicillin may be the most popular,which is the multidrug resistance pattern of major epidemic strains.These 8 antimicrobial drugs should be used carefully.