Drug-resistance of Methicillin-resistant Staphylococcus Aureus and Detection of Its Panton-Valentine Leukocidin Gene

OBJECTIVE:To study the resistance of methicillin-resistant Staphylococcus aureus(MRSA) and detect its Panton-Valentine leukocidin (PVL) gene. METHODS:45 strains of MRSA were clinically isolated from the patients in The First Hospital of Hebei Medical University from January 2007 to June 2008. The susceptibility of the MRSA strains to 17 kinds of antibiotics was tested by Kerby-Bauer disk diffusion test,and the PVL gene was detected by polymerase chain reaction(PCR) technology. RESULTS:The resistant rates of the 45 strains of MRSA to penicillin G,erythromycin,clindamycin,ciprofloxacin and tetracycline were all above 70%. PVL genes were found in 12 of the total 45 MRSA strains,with a detection rate of 26.7%. CONCLUSION:MRSA shows multi-drug resistance,and PVL gene is a key causative agent of MRSA.