Detection of Powdery Mildew Resistance Genes in Wheat Germplasm Using Molecular Markers

[Objective] In order to promote the use of the wheat powdery mildew resistance genes in wheat breeding and production.[Method] The distribution of Pm21,Pm30,and Pm34 genes in 124 wheat variety resources using STS,SCAR and SSR molecular markers was detected.[Result] The results showed that the frequency of Pm30 gene(16 wheat varieties,12.9%) was highest,Pm21 was higher(7 wheat varieties,5.65%) and Pm34 was lowest(4 wheat varieties,3.23%) in 124 wheat variety resources.What's more,the research showed that part of wheat variety resources contained two kinds of wheat powdery mildew resistance genes combination,and their distribution frequency was also different.One material(0.81%) was detected to contain Pm21 and Pm30.One(0.81%) carried Pm30 and Pm34.We did not find Pm21 + Pm34 two resistance genes aggregation form or Pm21+ Pm30 + Pm34 three types of resistance genes polymerization form.[Conclusion] We detected variety resources with powdery mildew resistance gene of Pm21 or Pm30 or Pm34 chaining specific PCR markers,so as to provide broad spectrum and durable resistance materials for wheat breeding.