Evaluation of the Known Wheat Powdery Mildew Resistance Genes in Henan Province and Marker Tracing of Pm2 Gene

In order to clarify the effectiveness of the known wheat powdery mildew resistance genes(Pm) in Henan province,39 wheat varieties carrying known Pm genes were artificially inoculated at the seedling stage by 7 isolates of powdery mildew from major wheat growing regions in Henan province.The results showed that Pm1,Pm3a,Pm3b,Pm3c,Pm3d,Pm3e,Pm5a,Pm7,Pm8 were susceptible to most of isolates.Pm2,Pm4b,Pm6,Pm12,Pm17,Pm18,Pm19,Pm22,Pm24 were susceptible to few of isolates.Pm3f,Pm4a,Pm5(Mli),Pm13,Pm16,Pm20,Pm21,Pm23 were high resistance to all isolates.The pyramids of powdery mildew resistance genes in Maris Dove,Normandie and Arthur were susceptible to all isolates,while CI12632 and Maris Huntsman displayed good resistance.SSR marker Xcfd81 was used to detect the above five gene combinations.This marker could not be identified in Maris Dove,Normandie and Arthur,it approved that Pm2 gene had lost in them.These results will be a very significant guidance to rational use of known genes in wheat resistant breeding.