Identification and Application of the Special Marker of Pm13 and Pm21 Genes with Resistance to Powdery Mildew in Wheat

50 wheat varieties(lines),the parent materials with Pm13 or Pm21 genes and their F1 plants were molecularly detected by using the special markers linked to Pm13 and Pm21 genes with powdery mildew resistance to provide breeding materials with wide resistance spectrum and lasting resistance for wheat production and to promote application of additive genes in powdery mildew resistance of wheat breeding.The results showed that the special bands with 564 bp or 140 bp were amplified in the materials with corresponding genes and 4 F1 plants with Pm13 and Pm21 genes by using the special markers linked to Pm13 and Pm21 genes with powdery mildew resistance respectively.10 varieties(lines) of 50 tested varieties have Pm21 gene and the marker band of Pm13 gene does not amplified in all 50 tested varieties(lines).The varieties(lines) with Pm13 or the special marker linked to Pm21 all show the strong resistance to wheat powdery mildew in fields,which indicates that the special markers linked to Pm13 and Pm21 genes can be effectively used in powdery mildew resistance of wheat breeding and the molecular marker is an effective mean for detecting additive of resistance genes and supplemental breeding.