Cell preparation and histological observation of bone marrow stem cell-derived hepatocytes in the cell transplantation

Objective To study the in vitro labeling method for the hepatocytes differentiated from bone marrow stem cells,and the histology of transplanted hepatoeytes in the liver.Methods Rat bone marrow cells were isolated and induced into mature hepatoeytes in vitro.These hepatocytes were in- corporated with 5-Bromo-2-deoxyuridine(BrdU)in the culture.The labeled cells were transplanted into partial hepatectomy recipient rats.The histology and function of donor hepatoeytes were monitored by im- munohistochemistry or immunofluorescenee.Results The specific brown staining was found in differentia- ted hepatocytes after BrdU incorporation.This staining was also detected in liver tissue slices after the liver transplantation.BrdU-stained cells showed positive albumin staining.Conclusion The bone marrow stem cells-differentiated hepatocytes were histologically and functionally stable after transplantation and are ideal source for liver transplantation.