2007•Zhonghua shiyan waike zazhiRequires access
Bone marrow-derived hepatocytes in mice after acute liver injury
Wang Hong-shan
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Abstract
Objective To investigate the bone marrow-derived hepatocytes in mice after acute liver injury.Methods After acute liver injury induced by carbon tetrachloroid and transplantation of bone marrow cells isolated from green fluorescence protein (GFP) transgenic mice within 24 h,murine liver tis- sues were collected and detected for bone marrow-derived hepatocytes by double immunofluorescence stai- ning.Results Hematopoietic stem cells derived from transplanted bone marrow cells existed in liver sinu- soid during 8 weeks.Immature bepatocytes expressing AFP differentiated from transplanted bone marrow cells were identified at 1,2,4,6 and 8 week after transplantation,while mature hepatocytes differentiated from transplanted bone marrow cells were observed at 4,6,8 week after transplantation.Conclusion Bone marrow-derlved hepatocytes can be identified after acute liver injury,and bone marrow may be one of the potential compartments for liver regeneration.