Surveillance of antimicrobial susceptibility of Neisseria gonorrhoeae and its plasmid analysis in Shanghai from 2001 to 2003

Objective To survey the susceptibility and trend of Neisseria gonorrhoeae (N.gonorrhoeae) strains to antibiotic agents in Shanghai from 2001 to 2003. Methods 742 N.gonorrhoeae strains from clinical patients were randomly collected and observed for its susceptibility to five antibiotics.Agar dilution method was used to detect minimal inhibitory concentration (MIC) of anti-microbial agents:penicillin,tetracycline,spectinomycin,ciprofloxacin and ceftriaxone.β-lactamase testing was perforomed by acidometric method.The plasmid DNA was extracted by alkaline cleavage method. Results 342(46.09%) strains were penicillinase-producing N. gonorrhoeae (PPNG);166(22.37%) strains were high-level tetracycline resistant N.gonorrhoeae (TRNG);718(96.77%) strains were resistant to ciprofloxacin;1 strain was resistant to spectinomycin;No resistant strain to ceftriaxone was found.Multi-resistant (20%) strains to penicillin,tetracycline and ciprofloxacin simultaneously were detected.35 strains were detected for plasmid map,34 trains showed 5.4 and 7.4 kb plasmid respectively. Conclusions The results indicate that at present,spectionmycin and ceftriaxone are the good choice for the treatment of gonorrhea.