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2015•Journal of Binzhou Medical UniversityRequires access

The effect of HS-1200 on the proliferation and the secretion of type I collagen of hepatic stellate cell

Yin Hongmi

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Abstract

Objective To research the effect of HS-1200 on the proliferation and the secretion of type I collagen of hepatic stellate cell by Induction of TGF-β1.Methods HSC-T6 cells were treated with different concentrations of HS-1200(60μM,40μM,20μM)for 24 hours and 48 hours,5ng/ml TGF-β1is added for stimulating.The effect of HS-1200 on HSC-T6 proliferation is analyzed with MTT colorimetry and cell periodic location is examined by Flow Cytometry(FCM);expressions of type I collagen and cyclin D1,cyclin E protein is examined by immunocytochemistry.Apoptosis of HSC-T6 is detected by DNA fragmentation.Results HS-1200 can remarkably inhibit the stimulation of TGF-β1to HSC-T6 proliferation and induce an increase of HSC-T6 in G_0/G_1 stage.Conclusion HS-1200 can remarkably inhibit the expression of type I collagen and induce apoptosis of HSC-T6 cells stimulated by TGF-β1.

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Objective To research the effect of HS-1200 on the proliferation and the secretion of type I collagen of hepatic stellate cell by Induction of TGF-β1.Methods HSC-T6 cells were treated with different concentrations of HS-1200(60μM,40μM,20μM)for 24 hours and 48 hours,5ng/ml TGF-β1is added for stimulating.The effect of HS-1200 on HSC-T6 proliferation is analyzed with MTT colorimetry and cell periodic location is examined by Flow Cytometry(FCM);expressions of type I collagen and cyclin D1,cyclin E protein is examined by immunocytochemistry.Apoptosis of HSC-T6 is detected by DNA fragmentation.Results HS-1200 can remarkably inhibit the stimulation of TGF-β1to HSC-T6 proliferation and induce an increase of HSC-T6 in G_0/G_1 stage.Conclusion HS-1200 can remarkably inhibit the expression of type I collagen and induce apoptosis of HSC-T6 cells stimulated by TGF-β1.

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Available abstract

Objective To research the effect of HS-1200 on the proliferation and the secretion of type I collagen of hepatic stellate cell by Induction of TGF-β1.Methods HSC-T6 cells were treated with different concentrations of HS-1200(60μM,40μM,20μM)for 24 hours and 48 hours,5ng/ml TGF-β1is added for stimulating.The effect of HS-1200 on HSC-T6 proliferation is analyzed with MTT colorimetry and cell periodic location is examined by Flow Cytometry(FCM);expressions of type I collagen and cyclin D1,cyclin E protein is examined by immunocytochemistry.Apoptosis of HSC-T6 is detected by DNA fragmentation.Results HS-1200 can remarkably inhibit the stimulation of TGF-β1to HSC-T6 proliferation and induce an increase of HSC-T6 in G_0/G_1 stage.Conclusion HS-1200 can remarkably inhibit the expression of type I collagen and induce apoptosis of HSC-T6 cells stimulated by TGF-β1.

Key concepts: Hepatic stellate cell, Flow cytometry, Cyclin D1, Apoptosis, Cell growth, Molecular biology, Cell cycle, Immunocytochemistry

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