Effects of Buddlejasaponin IV on Proliferation and Activation of Hepatic Stellate Cells

Objective: To investigate the effect of buddlejasaponin Ⅳ on proliferation and activation of rat hepatic stellate cell line HSC-T6.Method: HSC-T6 cells was incubated with buddlejasaponin Ⅳ at different concentrations(20,14,9.8,6.86 mg·L-1) for 24 hours.Effect of buddlejasaponin Ⅳ on proliferation of HSC-T6 was observed by MTT assay.Col-I and Col-Ⅲ mRNA expressions were assayed by reverse transcription ploymerase chain reaction(RT-PCR) after incubation.Apoptosis of HSC-T6 was detected by Annexin V/ PI staining combined with flow cytometry method.Result: Buddlejasaponin Ⅳ could inhibit HSC-T6 cell proliferation,in a dose-and time-dependent manner.Buddlejasaponin Ⅳ with 6.86 mg·L-1 inhibited Col-Ⅲ mRNA expression(P0.05).Buddlejasaponin Ⅳ with 9.8 mg·L-1 decreased Hyp secretion,inhibited Col-I mRNA expression(P0.05) and induced apoptosis significantly(P0.01),so as to achieve the effect of anti-hepatic fibrosis.Conclusion: Buddlejasaponin Ⅳcould interfere liver fibrosis via inhibiting proliferation and activation of HSC-T6 cells and inhibiting the synthesis of extracellular matrix.