2003•Chinese HepatologyRequires access
Influence of Salvianolic acid B on transformin growth factor-β_1 stimulated collagen secretion by hepatic stellate cells
Chenghai Liu
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Abstract
Objective To observe the influence of Salvianoic acid B(SA-B) on secretion of transforming growth factor-β 1(TGF-β 1) by hepatic stellate cells(HSC) and to investigate the collagen secretion stimulated by TGF-β 1. Methods The secretion of TGF-β 1 in primary and activated HSC were assayed by ELISA. The collagen secretion stimulated by TGF-β 1 was detected by [ 3H] proline incorporation and collagenase digestion, and collagen I expression was by Western blot. The SA-B was incubated with HSC and its effects on the collagen production and TGF-β 1 secretion were observed.Results The secretion of TGF-β 1 in activated HSC was much higher than that in the primary HSC. SA-B could markedly inhibit the secretion of TGF-β 1 in activated HSC and TGF-β 1 increased the collagen secretion and collagen I expression significantly.Conclusion SA-B can inhibit the secretion of TGF-β 1 in activated HSC, and decrease the collagen synthesis of HSC stimulated by TGF-β 1.