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RESEARCH ON THE OPTIMUM CONDITIONS FOR SOYBEAN CALLUS SUBCULTURE

Yusong Zhang, Kou Weicai

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Abstract

In this paper,the subculture of soybean were studied from the aspects of basic media,culturing conditions,phytohormones etc.The optimum conditions for soybean callus subculture were as follows: Growth status of callus was better on MS medium supplemented with BA0.5 mg/L,2,4-D2.0 mg/L,the callus yield and increasing times were the highest,it can reached 11.23 g/Flask and 10.23.The suitable pH value of medium was among 5.8~6.0.The callus cultured in light(1000~1500Lx,12 h/d) for 21d were suitable for the callus growth.

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What this paper is about

In this paper,the subculture of soybean were studied from the aspects of basic media,culturing conditions,phytohormones etc.The optimum conditions for soybean callus subculture were as follows: Growth status of callus was better on MS medium supplemented with BA0.5 mg/L,2,4-D2.0 mg/L,the callus yield and increasing times were the highest,it can reached 11.23 g/Flask and 10.23.The suitable pH value of medium was among 5.8~6.0.The callus cultured in light(1000~1500Lx,12 h/d) for 21d were suitable for the callus growth.

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Available abstract

In this paper,the subculture of soybean were studied from the aspects of basic media,culturing conditions,phytohormones etc.The optimum conditions for soybean callus subculture were as follows: Growth status of callus was better on MS medium supplemented with BA0.5 mg/L,2,4-D2.0 mg/L,the callus yield and increasing times were the highest,it can reached 11.23 g/Flask and 10.23.The suitable pH value of medium was among 5.8~6.0.The callus cultured in light(1000~1500Lx,12 h/d) for 21d were suitable for the callus growth.

Key concepts: Callus, Subculture (biology), Yield (engineering), Botany, Horticulture, Chemistry, Biology, Materials science

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