Extraction and Determination of Polysaccharide from Aloe
Lin Xin
Abstract
Lin Xin
Abstract
Objective To extract polysaccharide from Aloe. Methods The polysaccharide was purified by precipitation with ethanol followed by extraction with distilled water. Its total soluble sugar content was detected colorimetrically by the phenol\|sulfuric acid method with mannose as standard. \{Results\} The soluble sugar contents of gel, skin and whole leave were 0 997, 1 66 and 1 08 mg/g, \{respectively\}. Conclusion This method is accurate and well reproducible.
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Objective To extract polysaccharide from Aloe. Methods The polysaccharide was purified by precipitation with ethanol followed by extraction with distilled water. Its total soluble sugar content was detected colorimetrically by the phenol\|sulfuric acid method with mannose as standard. \{Results\} The soluble sugar contents of gel, skin and whole leave were 0 997, 1 66 and 1 08 mg/g, \{respectively\}. Conclusion This method is accurate and well reproducible.
Key concepts: Polysaccharide, Chromatography, Distilled water, Chemistry, Ethanol precipitation, Sugar, Extraction (chemistry), Sulfuric acid