Construction of survivin siRNA expression vector and its expression in esophagus carcinoma cell line EC109

[Objective] The aim of this study was to construct a siRNA expression plasmid against gene survivin.So we can assess its function on esophagus carcinoma cell EC109.[Methods] Potential RNAi oligonucleotides of survivin were selected on appropriate site.Then a survivin siRNA plasmid was constructed using a pBAsi-hU6 vector.The plasmid was sequenced to confirm the inserted sequence.Western blot analysis was taken to know about the change of survivin protein after the constructed plasmid had been transfected into EC109 cells.[Results] It was confirmed by sequencing that the plasmid had been constructed successfully.The result of Western blot showed that survivin siRNA plasmid had inhibited survivin expression in EC109 cells obviously.[Conclusion] The survivin siRNA plasmid was constructed successfully.Survivin protein expression of EC109 was inhibited by RNAi.