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Construction and effect identification of RNAi eukaryotic expression vectors of survivin

Yang Jun

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Abstract

Objective To investigate apoptosis effect of siRNA on survivin in Adenoid cystic carcinoma cells lines.Methods siRNA was designed and synthesized.Eukaryotic expression vector pGenesil-shRNA-survivin was constructed.Recombinant Plasmid was transfected into ACC-2 cells.The expression of survivin mRNA was detected by RT-PCR and cell apoptosis was detected by TUNEL.Results The recombinant plasmid was successfully constructed.The expression of survivin mRNA was obviously reduced.ACC-2 cells apoptosis was promoted.Conclusion The RNAi eukaryotic vector pGenesil-shRNA-survivin was constructed successfully.The RNAi inhibitory effect on theACC-2 cells is confirmed.

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What this paper is about

Objective To investigate apoptosis effect of siRNA on survivin in Adenoid cystic carcinoma cells lines.Methods siRNA was designed and synthesized.Eukaryotic expression vector pGenesil-shRNA-survivin was constructed.Recombinant Plasmid was transfected into ACC-2 cells.The expression of survivin mRNA was detected by RT-PCR and cell apoptosis was detected by TUNEL.Results The recombinant plasmid was successfully constructed.The expression of survivin mRNA was obviously reduced.ACC-2 cells apoptosis was promoted.Conclusion The RNAi eukaryotic vector pGenesil-shRNA-survivin was constructed successfully.The RNAi inhibitory effect on theACC-2 cells is confirmed.

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Available abstract

Objective To investigate apoptosis effect of siRNA on survivin in Adenoid cystic carcinoma cells lines.Methods siRNA was designed and synthesized.Eukaryotic expression vector pGenesil-shRNA-survivin was constructed.Recombinant Plasmid was transfected into ACC-2 cells.The expression of survivin mRNA was detected by RT-PCR and cell apoptosis was detected by TUNEL.Results The recombinant plasmid was successfully constructed.The expression of survivin mRNA was obviously reduced.ACC-2 cells apoptosis was promoted.Conclusion The RNAi eukaryotic vector pGenesil-shRNA-survivin was constructed successfully.The RNAi inhibitory effect on theACC-2 cells is confirmed.

Key concepts: Survivin, RNA interference, Transfection, Small hairpin RNA, Molecular biology, Apoptosis, Recombinant DNA, Expression vector

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