Effects of RNA interference technology on the expression of endogenous Survivin in human lung adenocarcinoma cells A549

Objective To provide experimental basis for the tumor gene silencing induced by Survivin. Methods The DNA sequence targeting human Survivin was inserted into the plasmid by RNA interference technology(RANi),the recombinant plasmid were constructed and transfected into A549 cells. Before and after transfection,the cell proliferation was observed by MTT ,the levels of Survivin mRNA and Survivin protein were detected by RT-PCR and Western Blot. Results The recombinant plasmid pGenesil1.1-Survivin were successfully constructed. The A549 cells transfected with recombinant plasmid showed a lower mRNA and protein expression of Survivin,compared with th control group,the inhibition rate were 65% and 71% respectively.The A549 cell proliferation was inhibited. Conclusion The recombinant plasmid constructed by RNAi can inhibit the protein expression and RNA transcription of endogenous Survivin.