Tissue Culture of Dandelio

With pot-cultured dandelion as test material, the leaf stalk and blade were used as explants for tissue culture. Addition of 300 mg/l of vitamin C into the culture medium was most effective to prevent callus from browning, and the medium MS + 0.5 mg/l 6-BA + 0.1 mg/l NAA is an optimum combination for direct regeneration. For indirect regeneration fragile calli were obtained from explants on the medium supplemented with 6-BA 0.5 mg/l and 2,4-D 0.5~1.0 mg/l. The medium 1/2 MS + NAA 0.1 mg/l was suitable for rooting.