The Anti-enzymatic Hydrolysis Properties of Tanned Collagen

In this experiment,the properties of anti-enzymatic hydrolysis of three kinds of tanned collagen with different amount of tanning agents were evaluated using different kind of proteases.After the tanned leather hydrolyzed by the enzymes,the hydroxyproline concentration in the solution and the shrinkage temperature of the treated collagen were tested.It was found that the tanned collagen with the vegetable tannin and the glutaraldehyde are more resistant to hydrolysis with the neutral enzyme than chrome ones;the destructive of acidic protease on chrome tanned leather and the destructive of alkaline protease on vegetable tanned leather are more severe;also,the glutaraldehyde tanned leather has a widespread resistance to all types of enzymatic hydrolysis.