Preparation of Cow Leather Collagen Polypeptide with Complex Enzyme

In the study,the preparation of cow leather collagen polypeptide with complex enzyme was discussed,using the cow leather collagen made in advance as the experimental raw material.With the hydrolysis degree of collagen as indicator,the addition sequence and proportion of the two kinds of enzyme,papain and trypsin,were determined.Meanwhile,the optimized interval of technical factors was obtained by single factor method and the optimized process parameters using the multi-enzyme complex preparation of collagen polypeptide was obtained by response surface method.The experimental results show that the optimal preparation conditions of collagen polypeptide from collagen protein were as follows:the proportion based on enzyme activity between papain and trypsin being 3∶ 1,with an addition amount of 650 U/mL of enzyme,the pH value of the enzymolysis medium being at 7.0,the enzymolysis temperature of 50 ℃,and the enzymolysis to be maintained for 7 h.The hydrolysis degree of cow leather collagen can reach 32.02% under the optimized conditions.