HPLC-ECD determination of six phenolic acids in Yiganshukang capsules

Objective:To establish an HPLC-ECD method for simultaneous determination of the contents of gallic acid,protocatechuic acid,chlorogenic acid,caffeic acid,vanillin acid and ferulic acid in Yiganshukang capsules. Method:The separation was performed on Hypersil ODS(4.6mm×250mm,5.0μm)by gradient elution with methanol-0.1% aqueous acetic acid(pH=3.50)(0min,2:98 ;17min,20:80 ;30min,90:10)as the mobile phase at a flow rate of 0.8 mL·min~(-1).The detection was done at 0.7 V and 30℃.The samples were filtered through 0.45μm membrane.Calibrations of peak area versus concentration were applied in the determination.Re- suits:The limits of detection(ng)were 1 for gallic acid,3 for protocatechuic acid,3 for chlorogenic acid,6 for caf- feic acid,1 for vanillin acid and 5 for ferulic acid.The average recoveries of six phenolic acids were between 98.7% -99.6% with RSDs of 2.1%-3.2%.Conclusion:The method is simple,rapid,reproducible and accurate.It can be used for the routine analysis of the six phenolic acids in Yiganshukang capsules.