Determination of Protocatechuic Acid,Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC

Objective:To establish the methods for determining protocatechuic acid,chlorgenic acid and caf-feic acid in Sangye dispensing granules by HPLC.Method: The column of Hypersil ODS2 C18 column(4.6 mm ×250 mm,5 μm) was used.The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution.The flow rate was 1.0 mL.min-1.The column temperature was at 20 ℃,the detection wavelength was at 258 nm,328 nm.Result: The average recovery of protocatechuic acid,chlorgenic acid and caffeic acid was 102.1%,101.1% and 101.5% respectively.Protocatechuic acid,chlorgenic acid and caffeic acid were linear in the range of 0.032 2-0.161 0 μg,0.125 6-1.256 μg and 0.035 1-0.351 μg respectively.Conclusion: The method is simple,accurate and can used to determine the content of the protocatechuic acid in mulberry formula granules.