The Clinical Study of Subtypes Detection of HPV for Different Age Patients with Cervical Intraepithelial Neoplasia II~III after Loop Electrosurgical Excision Procedure

Objective:To assess the clinical effectiveness and follow-up value by testing the clearance rate of human papillomavirus(HPV) after loop electrosurgical excision procedure(LEEP) treatment in different age patients with cervical intraepithelial neoplasiaⅡ~Ⅲ(CINⅡ~Ⅲ).Methods:336 cases with CINⅡ~Ⅲ underwent loop electrosurgical excision procedure were enrolled.All patients were randomly assigned into three groups with each of 101 cases,according to different age,which were proved to be high risk HPVDNA infection preoperatively.Thin Prep liquid based cytology test(TCT) and HPVDNA detection were performed at 6th month postoperatively.The biopsies were done if necessary.The clearance and residual rate of HPVDNA were compared between groups.Results:① The clearance rate of three groups after Leep was 93.8%,71.4% and 64.7% respectively.The clearance rate of HPV after Leep treatment was higher in the group ranging from 21 to 30 years old than that in the latter two groups(P0.05).There was no statistical difference between the latter two groups(P0.05).②There were statistical differences in the group of 41 to 50 years old groups in persistent HPV16 infection,when compared to the group of 21 to 30 years old groups(P0.05).However there was no statistical difference in persistent HPV16 and other HPV subtypes infections(P0.05).③The residual rate of persistent HPV16 infection in 41 to 50 years old group was higher as compared to 21 to 30 years old group(P0.05).Conclusions:Leep is a simple and safe method that can effectively resect cervical lesion while clear HPV.We suggest the follow up patients by six-month repeat HPV subtypes detection for the women after Leep.Also,it is reasonable to propose a longer and closer fellow-up in 41 to 50 years old age population with persistent HPV16 infection after Leep.