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2013•Zhongguo shiyan fangjixue zazhiRequires access

Content Determination of Berberine Hydrochloride in Prossing Calamina by Sanhuang Tang

Liang Wang

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Abstract

Objective: To establish a method for the content determination of of berberine hydrochloride in prossing Calamina by Sanhuang Tang.Method: HPLC was used with lichrospher C18column(4.6 mm × 150 mm,5μm),acetonitrile-0.1% phosphoric acid(46∶ 64) with mobile phase.The flow rate was 1 mL.min-1;the column temperature was maintained at 30 ℃;the detection wavelength was set at 350 nm.Result: Berberine hydrochloride had a good linearity within 0.042 0-0.525 μg(r = 0.999 8) and the average recovery was 95.24% with RSD 1.33%.The content of berberine hydrochloride in three samples of prossing Calamina by Sanhuang Tang was 0.326%,0.347%,0.373%.Conclusion: This method is simple,accurate with good reproducibility,and can be used for the content determination of berberine hydrochloride in prossing Calamina by Sanhuang Tang.

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Objective: To establish a method for the content determination of of berberine hydrochloride in prossing Calamina by Sanhuang Tang.Method: HPLC was used with lichrospher C18column(4.6 mm × 150 mm,5μm),acetonitrile-0.1% phosphoric acid(46∶ 64) with mobile phase.The flow rate was 1 mL.min-1;the column temperature was maintained at 30 ℃;the detection wavelength was set at 350 nm.Result: Berberine hydrochloride had a good linearity within 0.042 0-0.525 μg(r = 0.999 8) and the average recovery was 95.24% with RSD 1.33%.The content of berberine hydrochloride in three samples of prossing Calamina by Sanhuang Tang was 0.326%,0.347%,0.373%.Conclusion: This method is simple,accurate with good reproducibility,and can be used for the content determination of berberine hydrochloride in prossing Calamina by Sanhuang Tang.

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Available abstract

Objective: To establish a method for the content determination of of berberine hydrochloride in prossing Calamina by Sanhuang Tang.Method: HPLC was used with lichrospher C18column(4.6 mm × 150 mm,5μm),acetonitrile-0.1% phosphoric acid(46∶ 64) with mobile phase.The flow rate was 1 mL.min-1;the column temperature was maintained at 30 ℃;the detection wavelength was set at 350 nm.Result: Berberine hydrochloride had a good linearity within 0.042 0-0.525 μg(r = 0.999 8) and the average recovery was 95.24% with RSD 1.33%.The content of berberine hydrochloride in three samples of prossing Calamina by Sanhuang Tang was 0.326%,0.347%,0.373%.Conclusion: This method is simple,accurate with good reproducibility,and can be used for the content determination of berberine hydrochloride in prossing Calamina by Sanhuang Tang.

Key concepts: Berberine hydrochloride, Berberine, Chromatography, Chemistry, Phosphoric acid, Content determination, High-performance liquid chromatography, Hydrochloride

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