DETERMINATION OF FERULIC ACID,VANILLIC ACID AND VANILLIN SIMULTANEOUSLY BY TLC IN BIOTRANSFORM PROCESS

The contents of ferulic acid,vanillic acid and vanillin in fermentation liquid can be determined simultaneously by TLC,with nhexanechloroformdehydrated etherglacial acetic acid (4∶3∶2∶01) as devekoper and iodine vapor as colordeveloping agent.Vanillin can be determined with 2,4nitrophenyhydrazine as colordeveloping agent specially.The linear range of feruli acid,vanillic acid and vanillin are all between 1～10 μg,their standard curve are y=3533 x-1832,R2=0995 4;y=3080 x-420,R2=0997 9;y=65511 x-490,R2=0995 4 respectively.The average recovery are 1013%,1018% and 1030% respectively,RSD are 239% and 224%.The method of using 2,4nitrophenyhydrazine is more sensitive.The linear range is 1～5 μg,and the standard curve is y=6 8436 x-477 6,R2=0992 2.The determination result of vanillin is same by these two methods.