Determination of vanillin And ethyl vanillin in dairy products by solvent extraction and gas chromatography

[Objective]To establish a method for determination of vanillin And ethyl vanillin in dairy products by solvent extraction and gas chromatography.[Methods]The improved method for determination of vanillin and ethyl vanillin in dairy products by solvent extraction was studied,as well as the analysis of conditions and detection method of gas chromatography.[Results] The linear relationship about vanillin and ethyl vanillin was good.Linear equations were Y=8.494 81e-1 ×X-3.268 92,r =0.998 6 and Y=9.947 50 e-1 ×X-2.655 43,r=0.999 3.The limit of detection was low.Vanillin and ethyl vanillin detection limits were 2.24 mg/kg and 3.13 mg/kg.The repeatability of method was good Detection of the relative standard deviations were generally ranged between 0.43%-8.13%.The recoveries of detection were 82%-93%.[Conclusion]Solvent extraction and gas chromatography analysis of vanillin and ethyl vanillin in dairy products has good accuracy and high precision.The method has obtained satisfactory results in determining vanillin and ethyl vanillin in 18 batches of dairy products.