Influence of oridonin on EC9706 cell proliferation and apoptosis

Aim:To study the influence of oridonin on esophageal squamous cell carcinoma EC9706 cell proliferation and apoptosis in vitro. Methods:MTT assay was used to detect the inhibition effect of oridonin on the growth of EC9706; flow cytometry was applied to detect the impact of oridonin on cell cycle and apoptosis of EC9706,and calculated the apoptosis rate of cells; change of Ca2 +fluorescence intensity of EC9706 affected by different concentrations of oridonin was observed by laser scanning confocal microscope. Results:MTT assay showed that the inhibition rate in the experiment group increased with the rising of concentration( F = 370. 600,P 0. 001) after oridonin acting on EC9706; results of cell cycle detected by flow cytometry showed that the number of cell in G 0 and G 1 stage increased notably after oridonin acting on EC9706 for 48 hour respectively( F = 24. 200,P 0. 001); results of apoptosis showed that oridonin could obviously inducethe apoptosis of EC9706( P 0. 05),and the effect was the best when the oridonin concentration was 40 μmol/L( F = 10. 214,37. 820,P 0. 001). Different concentrations of oridonin could increase Ca2 +fluorescence intensity of EC9706, and the difference was significant( F = 24. 400,P 0. 001). Conclusion:oridonin can improve the inhibition effects on proliferation and apoptosis of EC9706 cell.