2001•Wuxi Qinggong Daxue xuebaoRequires access

Determination of Lycopene in Tomato Oleoresin by HPLC

Qing Sun

Abstract

The content of lycopene in tomato oleoresin was determined by HPLC. The results showed that the best mobile phase was acetonitrile: methanol: dichloromethane (7∶7∶2) detected at 472nm. The retention times of lycopene and beta carotene were 9～10 minutes and 13～14 minutes respectively. The reproductive SD of lycopene was lower than 5 percent and the recovery rate was 98～101 percent.

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Key concepts: Lycopene, Oleoresin, High-performance liquid chromatography, Dichloromethane, Chromatography, Chemistry, Acetonitrile, Carotenoid