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2012•Zhongguo gua-caiRequires access

Measurement of Lycopene Contents in Watermelon

Yuan Ping-li, Lu Xuqiang, Wenge Liu, Zhao ShengJie, Lu Jinsheng, Zhihong Yan, Nan He, Guan Li-ying, Zhu HongJu

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Abstract

Watermelon(Citrullus lanatus)is one of the few species that accumulate a large amount of lycopene in fruits with health-benefits to human. The contents of lycopene in different ploidy watermelon fruits were determined by HPLC and UV-visible spectrophotometric methods to develop an accurate and convenient method for watermelon lycopene content measurement. The HPLC method was simple and accurate with the mobile phase methanol∶ acetonitrile:dichloromethane at ratio of 20 ∶ 75 ∶ 5 and detected at 502 nm. The amount of lycopene measured by U. V. visible spectrophotometric method was 1.45 times of that by HPLC. Both methods can be used to compare lycopene content in different watermelon cultivars. In addition, our results indicated that polyploidy watermelons have much more lycopene than diploid one.

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What this paper is about

Watermelon(Citrullus lanatus)is one of the few species that accumulate a large amount of lycopene in fruits with health-benefits to human. The contents of lycopene in different ploidy watermelon fruits were determined by HPLC and UV-visible spectrophotometric methods to develop an accurate and convenient method for watermelon lycopene content measurement. The HPLC method was simple and accurate with the mobile phase methanol∶ acetonitrile:dichloromethane at ratio of 20 ∶ 75 ∶ 5 and detected at 502 nm. The amount of lycopene measured by U. V. visible spectrophotometric method was 1.45 times of that by HPLC. Both methods can be used to compare lycopene content in different watermelon cultivars. In addition, our results indicated that polyploidy watermelons have much more lycopene than diploid one.

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Available abstract

Watermelon(Citrullus lanatus)is one of the few species that accumulate a large amount of lycopene in fruits with health-benefits to human. The contents of lycopene in different ploidy watermelon fruits were determined by HPLC and UV-visible spectrophotometric methods to develop an accurate and convenient method for watermelon lycopene content measurement. The HPLC method was simple and accurate with the mobile phase methanol∶ acetonitrile:dichloromethane at ratio of 20 ∶ 75 ∶ 5 and detected at 502 nm. The amount of lycopene measured by U. V. visible spectrophotometric method was 1.45 times of that by HPLC. Both methods can be used to compare lycopene content in different watermelon cultivars. In addition, our results indicated that polyploidy watermelons have much more lycopene than diploid one.

Key concepts: Lycopene, Citrullus lanatus, High-performance liquid chromatography, Carotenoid, Cultivar, Biology, Dichloromethane, Food science

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