2007•Chemistry & BioengineeringRequires access

Simultaneous Determination of Vanillic Acid,Ferulic Acid and Cinnamic Acid in Picrohiza Scrophulariiflora Pennell by HPLC

Jianxun Liu

Abstract

The simultaneous determination of vanillic acid,ferulic acid and cinnamic acid in Picrohiza scrophulariiflora Pennell by RP-HPLC method was reported in this paper.The HPLC separation was performed on a Shim-pack C18-ODS column(250 mm×4.6 mm,5 μm) with mobile phase of acetonitrile-0.05% phosphoric acid(13∶87) and detected at wavelength 290 nm.The calibration curve was linear in the range of 14.0～210.0 ng for vanillic acid(R=0.9996),52.0～520.0 ng for ferulic acid(R=0.9995)and 6.0～90.0 ng for cinnamic acid(R=0.9993).The average recovery of vanillic acid,ferulic acid and cinnamic acid was 96.6%,98.9% and 97.4%,respectively.The method was accurate with a good reproducibility.

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Key concepts: Vanillic acid, Ferulic acid, Chemistry, Cinnamic acid, Chromatography, Phosphoric acid, High-performance liquid chromatography, Calibration curve