Strain idendification of Porphyromonas gingivalis by sequencing analy sis of ribosomal intergenic spacer region

Objective To set up a method for stra in identification of Porphyromonas gingivalis(Pg)by sequencing analysis of ribosom al intergenic spacer region.Methods Genomic DNA isolated from laboratory strain(Pg)wa s used as a template for PCR.PCR products with ribosomal intergenic spacer regio n were purified via the geneclean protocol and sequenced with Ampli cycle TM seq uencing Kit for radioactive sequencing reactions.Results The amplified products appeared at approximatery 0.88kb.The base sequence of ribosomal intergenic spacer region had been determin ed.Conclusion Ribosomal intergenic spacer region sequencing analysis provides a strain identifier for Pg that can be easily reproduced among laboratories.