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Cloning and Sequencing of part of 16S rRNA gene of Porphyromonas gingivalis

Han Su-feng

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Abstract

AIM:To clone and analyse part of the sequence of 16S ribosomal RNA(16S rRNA) gene of Porphyromonas gingivalis(Pg ).METHODS:Polymerase chain reaction(PCR) was used to generate part of the 16S rRna gene of Pg ,then the gene was cloned and sequence analysed.RESULTS:A segment of DNA,size of which was 198 bp,was obtained and sequenced.The sequence was consistent with that displayed in Genbank on pubmed.CONCLUSION:Part of 16S rRNA gene was generated by PCR that could be the foundation of detection of Pg .

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What this paper is about

AIM:To clone and analyse part of the sequence of 16S ribosomal RNA(16S rRNA) gene of Porphyromonas gingivalis(Pg ).METHODS:Polymerase chain reaction(PCR) was used to generate part of the 16S rRna gene of Pg ,then the gene was cloned and sequence analysed.RESULTS:A segment of DNA,size of which was 198 bp,was obtained and sequenced.The sequence was consistent with that displayed in Genbank on pubmed.CONCLUSION:Part of 16S rRNA gene was generated by PCR that could be the foundation of detection of Pg .

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Available abstract

AIM:To clone and analyse part of the sequence of 16S ribosomal RNA(16S rRNA) gene of Porphyromonas gingivalis(Pg ).METHODS:Polymerase chain reaction(PCR) was used to generate part of the 16S rRna gene of Pg ,then the gene was cloned and sequence analysed.RESULTS:A segment of DNA,size of which was 198 bp,was obtained and sequenced.The sequence was consistent with that displayed in Genbank on pubmed.CONCLUSION:Part of 16S rRNA gene was generated by PCR that could be the foundation of detection of Pg .

Key concepts: 16S ribosomal RNA, GenBank, Porphyromonas gingivalis, Gene, Biology, Ribosomal RNA, Polymerase chain reaction, Cloning (programming)

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