Molecular cloning and DNA sequencing of 5s-23srRNA intergenic spacer DNA of four strainBorrelia burgdorferi isolated in Guangxi.

objective To investigate the Genetic characterization in Guangxi′s isolate of Borrelia bur_gdorferi.Methods PCR technique was used to amplify the_s_23srRNA intergenic spacer DNA from the whole cellular DNA of isolate GXLD_4,9,18 and Chang14.The amplified products were cloned into plasmid PAGE_T and sequenced. Results The 5s23srRNA intergenic spacer DNA of the four isolates were 242bp,revealed a nucletide sequence homology of99%.The four isolates had higher homology of nucleotidessequence with B.valaisiana than other genotypes.Conclusion The four isolates belong to B.valaisiana genotypes.This is a first report in china.