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2002•Journal of Qingdao UniversityRequires access

A RAPID DETECTION FOR BENZOIC ACID,SORBIC ACID AND SACCHAR IN FOOD BY HPLC TECHNIQUE

Mu ZhiChun

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Abstract

To develop high pressure liquid chromatography(HPLC) as a rapid detection technique for benzoic acid,sorbic acid and saccharin in food,an optimized chromatographic condition of a Luna C18 stainless column(150.0 mm×4.6 mm×10 μm)and methanol:0.02 mol/L NH 4Ac(33.5∶66.5,V∶V)as a mobile phase were used.The HPLC technique was improved to be a rapid detection system.The time required for analyzing one sample shortened from 20 minutes to within 4 minutes.The three components determined were not influenced,the extraneous peaks and resolution rates among them were free from considerable decrease(R1.5).There was a good linear correlation and relatively lower detection limits in the range of 0-100 mg/L.The rate of recovery of sample addition was 92%-102%?

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What this paper is about

To develop high pressure liquid chromatography(HPLC) as a rapid detection technique for benzoic acid,sorbic acid and saccharin in food,an optimized chromatographic condition of a Luna C18 stainless column(150.0 mm×4.6 mm×10 μm)and methanol:0.02 mol/L NH 4Ac(33.5∶66.5,V∶V)as a mobile phase were used.The HPLC technique was improved to be a rapid detection system.The time required for analyzing one sample shortened from 20 minutes to within 4 minutes.The three components determined were not influenced,the extraneous peaks and resolution rates among them were free from considerable decrease(R1.5).There was a good linear correlation and relatively lower detection limits in the range of 0-100 mg/L.The rate of recovery of sample addition was 92%-102%?

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Available abstract

To develop high pressure liquid chromatography(HPLC) as a rapid detection technique for benzoic acid,sorbic acid and saccharin in food,an optimized chromatographic condition of a Luna C18 stainless column(150.0 mm×4.6 mm×10 μm)and methanol:0.02 mol/L NH 4Ac(33.5∶66.5,V∶V)as a mobile phase were used.The HPLC technique was improved to be a rapid detection system.The time required for analyzing one sample shortened from 20 minutes to within 4 minutes.The three components determined were not influenced,the extraneous peaks and resolution rates among them were free from considerable decrease(R1.5).There was a good linear correlation and relatively lower detection limits in the range of 0-100 mg/L.The rate of recovery of sample addition was 92%-102%?

Key concepts: Sorbic acid, Chromatography, Benzoic acid, High-performance liquid chromatography, Chemistry, Saccharin, Detection limit, Methanol

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A RAPID DETECTION FOR BENZOIC ACID,SORBIC ACID AND SACCHAR IN FOOD BY HPLC TECHNIQUE — Research Paper | ScholarLens