[Simultaneous determination of 6 kinds of food additives in drink by HPLC-MS/MS].

OBJECTIVE: To establish a high performance liquid chromatographytandem mass spectrometry( HPLC-MS/MS) method for simultaneous determination of caffeine, benzoic acid, sorbic acid, acesulfame potassium, saccharin sodium and sodium cyclamate in drink. METHODS: The sample was extracted by ASPEC using extraction column( Pro Elut C_(18)500 mg/6 m L), dried by Turbo VapⅡ and dissolved in mobile phase. The supernatant was separated on Agilent SB-C_(18) column( 2. 1 mm × 50 mm, 1. 8 μm)using 0. 02 mmol/L ammonium acetate/methyle alcohol as mobile phase and then detected by HPLC-MS/MS using multiple reaction monitoring( MRM) in positive ionization and negative ionization mode. RESULTS: The average recoveries were from 72. 6% to 100. 5%and the relative standard deviations( RSD) were from 0. 3% to 3. 1%( n = 6). The calibration curves showed a good linearity with correlation coefficients r > 0. 9990. The linear range for saccharin sodium was 8-800 μg/L and others were 2-200 μg/L. The detection limits for caffeine, benzoic acid, sorbic acid, acesulfame potassium and sodium cyclamate were 0. 001 mg/kg and 0. 004 mg/kg for saccharin sodium. CONCLUSION: The method is specific, sensitive, easy, fast and suitable for the confirmation and quantification of caffeine, benzoic acid, sorbic acid, acesulfame potassium, saccharinsodium and sodium cyclamate in drink.