Comparative Study on Two methods of DNA Extraction from Pepper

CTAB and SDS method were adopted to extract DNA from fresh pepper leaves.Nucleic acid protein detector method,agarose gel electrophoresis method and PCR amplification methods were applied to measure the DNA samples.Nucleic acid protein detector measurement showed OD260/OD280 extracted with SDS method was above 2.0,which means protein has been wiped out completely while RNA has not been wiped out completely yet;OD260/OD280 extracted with CTAB method range from 1.8 to 2.0,which means protein,polysaccharides and RNA have been wiped out completely.Agarose gel electrophoresis measurement showed DNA extracted with SDS method has polysaccharides and RNA which failed to be removed and other secondary metabolites,while polysaccharides,RNA and other secondary metabolites can not be found in DNA extracted with CTAB method.PCR amplification measurement shows both DNA extracted with SDS method and DNA extracted with CTAB method can be amplified and used for SRAP research,but samples extracted with CTAB have clearer amplification band.The results showed DNA extracted with CTAB has high content,purity,and it can satisfy the needs of research on molecular biology.