Apolipoprotein E polymorphism and plasma lipid levels in obesity children in Baotou

OBJECTIVE To investigate ApoE polymorphism and plasma lipid levels in obesity children. METHODS We examined the ApoE genotypes and plasma lipids of 60 obese children(60 control children)from two middle schools aging from 12 to14 years old. ApoE genotyping was performed by polymerase chain resection-restriction fragment length polymorphism(PCR-RFLP). RESULTS In our research,4 genotypes of ApoE gene(E3/3,E3/2,E3/4 and E2/4)were detected. The allele frequency distribution was similar between the two groups and e3 allele frequency distribution was the highest. Compared with control subjects,e4 allele frequency distribution was higher(P﹤0.05). In obese group,TC,TG and LDL-C levels were E2/X﹤E3/3﹤E4/X(P﹤0.05),HDL-C level was E2/X﹥E3/3(P﹤0.05). In controls,TC level was the same in obesity group,and LDL-C level was E2/X﹤E3/3(P﹤0.05). CONCLUSION The results demonstrated that ApoE polymorphism did influence plasma lipid levels of obese children in our region.