Study on ~(60)Co-γ irradiation mutation breeding of kojic acid production strain and its characterization

Asperqillus oryzae were irradiated by different doses of 60Co-γ rays to obtain high-yielding strains of kojic acid.The results indicated that,when the dose of the 60Co-γ rays was 800Gy,it could have the best mutation effect to the start strain.The kojic acid yield of the mutant increased from original 15.68g/L to 61.5g/L,increased by 292.2%.It had a good genetic stability and stably acid producing.To obtain the characteristics changes of the C8-128 which has a high yielding of kojic acid,it was compared to the starting strain in colony morphology and fermentation characteristics in flask.