Compound Mutation Breeding of Kojic Acid Productive Strain

Mutant(UR28) of producing high-yield Kojic acid was screened from productive strain Aspergillus oryzae w-56 after treated with UV two times, γ-ray of60Co two times. The period of fermentation was shorten to 68h from 130h. The Kojic acid production level reached up to 68g/L, increasing by 88.9% compared with original strain's 36g/L. The experiments showed that compounding mutation can alter original strains sensitivity to mutagnic agents, increase mutation frequency and raise kojic acid yield.