Expression of T-lymphocyte subsets and its clinical significance in aplastic anemia

Objective To investigate the changes of T-lymphocyte subsets,tumor necrosis factor(TNF-α) and HLA-DR before and after the treatment of aplastic anemia. Methods 38 cases with aplastic anemia from 2008 to 2010 in our hospital were selected as the study group,while 30 healthy subjects were chosen as the control group. T-lymphocyte subsets and HLA-DR,TNF-α in the two groups were detected by Flow Cytometry(FCM) and ELISA,respectively. Results Before the treatment,the serum CD4+ and CD4+/CD8+ level in the study group were significantly lower than that in the control group(P0.01) ,and the CD8+,HLA-DR and TNF-α level were significantly higher(P0.01) . After the treatment,the CD4+ and CD4+/CD8+ level in the study group increased and the CD8+,HLA-DR and TNF-α level decreased,with statistically significant difference compared with that before the treatment(P0.05) . Conclusion The maladjustment of immune system is closely related to the incidence of aplastic anemia,exibiting disbalance of T-lymphocyte subsets and abnormal activation of T cells.