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2007•Journal of Beijing University of Traditional Chinese MedicineRequires access

Content determination of puerarin from effective fraction of Naomaitong Formula

Shengwang Liang

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Abstract

Objective To establish the method for determining puerarin content from effective fraction of Naomaitong Formula.Method The chromatographic column was Hypersil ODS2 C18 (250 mm×4.6 mm,5 μm), the mobile phase was methanol to water (30∶70) with a flow velocity of 1.0 ml/min, and the detection wavelength was 250 nm in the phased-high performance liquid chromatography (RP-HPLC) procedure.Result The average recovery of puerarin was 99.72% (RSD=1.49%).Conclusion This procedure, which is easy and accurate, can be used as a quality control method for the effective fraction of Naomaitong Formula.

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What this paper is about

Objective To establish the method for determining puerarin content from effective fraction of Naomaitong Formula.Method The chromatographic column was Hypersil ODS2 C18 (250 mm×4.6 mm,5 μm), the mobile phase was methanol to water (30∶70) with a flow velocity of 1.0 ml/min, and the detection wavelength was 250 nm in the phased-high performance liquid chromatography (RP-HPLC) procedure.Result The average recovery of puerarin was 99.72% (RSD=1.49%).Conclusion This procedure, which is easy and accurate, can be used as a quality control method for the effective fraction of Naomaitong Formula.

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Available abstract

Objective To establish the method for determining puerarin content from effective fraction of Naomaitong Formula.Method The chromatographic column was Hypersil ODS2 C18 (250 mm×4.6 mm,5 μm), the mobile phase was methanol to water (30∶70) with a flow velocity of 1.0 ml/min, and the detection wavelength was 250 nm in the phased-high performance liquid chromatography (RP-HPLC) procedure.Result The average recovery of puerarin was 99.72% (RSD=1.49%).Conclusion This procedure, which is easy and accurate, can be used as a quality control method for the effective fraction of Naomaitong Formula.

Key concepts: Puerarin, Chromatography, Chemistry, High-performance liquid chromatography, Fraction (chemistry), Medicine, Alternative medicine, Pathology

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