Plant Regeneration using Immature Embryo from Ten Genotypes of Guizhou Maize via Embryogenic Callus

Immature embryos of ten maize(Zea mays L.) genotypes from Guizhou Province were used to induce embryogenic callus.Effects of genotypes and 2,4-D concentrations on the induction of embryogenic callus,as well as the regeneration ability were investigated in present work.The results showed that the optimal 2,4-D concentration for embryogenic callus induction were different depending on the genotypes.Addition of 1.0mg/L 2,4-D gave the best effect in Qiandan19,Qiandan20,Qiandan23,09H313,Daqing233,the parent of Yiyu 6 and the male parent of Yiyu6,however the optimal concentrations for the parent of Zunyu and Qiandan21 were 2.0mg/L and 3.0mg/L,respectively.Also,induction and differentiation of embryogenic callus diversified among the genotypes.The best induction rate of embryogenic callus were investigated from the parent of Zunyu(81.87%) and Daqing233(71.39%),and the highest rate of differentiation were obtained from Qiandan23(89.80%) and Qiandan21(80.00%).Among the ten genotypes,Qiandan23,Qiandan21 and the male parent of Yiyu6 demonstrated the higher regeneration ability,thus may facilitate transformation via agrobacterium.