2010•The Chinese Journal of Modern Applied PharmacyRequires access

Analysis of Tanshinone Constituents in Radix et Rhizoma Salviae Miltiorrhizae by HPLC-MS(n)

Fang Yuan

Abstract

OBJCTIVE To search the ESI-MS(n) performance of tanshinone constituents in Radix et Rhizoma Salviae Miltiorrhizae and to establish its LC-MS(n) characteristic spectrums.METHODS Direct injection ESI-MS(n) has been used in the construction analysis of tanshinone ⅡA.Tanshinone constituents in Radix et Rhizoma Salviae Miltiorrhizae were studied by HPLC-MS(n).MS(n) was carried out on positive ion mode and HPLC was performed on a Zorbax SB-C18 column using gradient elution with a mixture of purified water and acetonitrile at the detection wavelength of 254 nm.RESULTS We got the proposed ESI-MS fragmention pathway of tanshinone constituents.By analyze of HPLC/ESI/MS(n) and HPLC/UV chromatograms,11 characteristic peaks were determined.CONCLUSION Tanshinone constituents have similar ESI-MS(n) performance that can be used in the structure identification of tanshinone compounds.HPLC-MS(n) is a useful tool for a special identification of tanshinone constituents in Radix et Rhizoma Salviae Miltiorrhizae.

About this research paper

What this paper is about

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Radix (gastropod), Chemistry, Chromatography, High-performance liquid chromatography, Gradient elution, Salvia miltiorrhiza, Traditional Chinese medicine, Biology