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Determination of Tanshinone II_A in Radix Et Rhizoma Salviae Miltiorrhizae Microemulsion by HPLC

Mao Deng, Yang Jian

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Abstract

Objective:To establish the determination method of Tanshinone Ⅱ_A in Radix Et Rhizoma Salviae Miltiorrhizae microemulsion by HPLC.Methods:The analysis was performed on a Diamonsil-C_(18) column(4.6 mm×250 mm,5 μm),with a mobile phase of methanol-water(80∶20).The flow rate was 1 mL·min~(-1).The detection wavelength was 270 nm.The column temperature was 35 ℃.Results: The calibration curve was linear in the range of(7.59～303.44)ng,r=(1.000 0).The accuracy,repeatability,stability and recovery of this method were satisfied.Conclusions:The established method of determinating for the content of Tanshinone Ⅱ_A in Radix Et Rhizoma Salviae Miltiorrhizae microemulsion was convenient,accurate and reliable.

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What this paper is about

Objective:To establish the determination method of Tanshinone Ⅱ_A in Radix Et Rhizoma Salviae Miltiorrhizae microemulsion by HPLC.Methods:The analysis was performed on a Diamonsil-C_(18) column(4.6 mm×250 mm,5 μm),with a mobile phase of methanol-water(80∶20).The flow rate was 1 mL·min~(-1).The detection wavelength was 270 nm.The column temperature was 35 ℃.Results: The calibration curve was linear in the range of(7.59～303.44)ng,r=(1.000 0).The accuracy,repeatability,stability and recovery of this method were satisfied.Conclusions:The established method of determinating for the content of Tanshinone Ⅱ_A in Radix Et Rhizoma Salviae Miltiorrhizae microemulsion was convenient,accurate and reliable.

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Available abstract

Objective:To establish the determination method of Tanshinone Ⅱ_A in Radix Et Rhizoma Salviae Miltiorrhizae microemulsion by HPLC.Methods:The analysis was performed on a Diamonsil-C_(18) column(4.6 mm×250 mm,5 μm),with a mobile phase of methanol-water(80∶20).The flow rate was 1 mL·min~(-1).The detection wavelength was 270 nm.The column temperature was 35 ℃.Results: The calibration curve was linear in the range of(7.59～303.44)ng,r=(1.000 0).The accuracy,repeatability,stability and recovery of this method were satisfied.Conclusions:The established method of determinating for the content of Tanshinone Ⅱ_A in Radix Et Rhizoma Salviae Miltiorrhizae microemulsion was convenient,accurate and reliable.

Key concepts: Radix (gastropod), Chromatography, Repeatability, Microemulsion, High-performance liquid chromatography, Chemistry, Calibration curve, Detection limit

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